AKR1C2 ELISA Kit (Human) (OKCD09448)

Catalog No: OKCD09448

Size:96WELLS

AKR1C2 ELISA Kit (Human) (OKCD09448) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrodiol Dehydrogenase 2 (DDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dihydrodiol Dehydrogenase 2 (DDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydrodiol Dehydrogenase 2 (DDH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydrodiol Dehydrogenase 2 (DDH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AKR1C2 Microplate: 96 Wells (12 x 8 Well strips)
  • AKR1C2 Lyophilized Standard: 2
  • 100X AKR1C2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: AKR1C2
• Gene Full Name: aldo-keto reductase family 1 member C2
• Alias Symbols: 3-alpha-HSD3;AKR1C-pseudo;aldo-keto reductase family 1 member C2;BABP;chlordecone reductase homolog HAKRD;DD;DD/BABP;DD2;DD-2;DDH2;Dihydrodiol dehydrogenase 2;dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III;Dihydrodiol dehydrogenase/bile acid-binding protein;HAKRD;HBAB;MCDR2;pseudo-chlordecone reductase;SRXY8;TDD;testicular 17,20-desmolase deficiency;trans-1,2-dihydrobenzene-1,2-diol dehydrogenase;type II dihydrodiol dehydrogenase;Type III 3-alpha-hydroxysteroid dehydrogenase.
• NCBI Gene Id: 1646
• Protein Name: Aldo-keto reductase family 1 member C2
• Description of Target: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability.
• Uniprot ID: P52895
• Protein Accession #: NP_001128713.1
• Nucleotide Accession #: NM_001135241.2