AKR1C1 Antibody (OAGA00409)

Catalog No: OAGA00409

Size:100 ul

AKR1C1 antibody detects AKR1C1 protein by Western blot analysis.
A. 50 ug mouse liver lysate/extract
10 % SDS-PAGE
AKR1C1 antibody (OAGA00409) dilution: 1:1000

Sample(30 ug whole cell lysate)
A:Raji
10% SDS PAGE
OAGA00409 diluted at 1:1000

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (OAGA00409) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using AKR1C1(OAGA00409) antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(OAGA00409) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid 1XPBS, 20% glycerol (pH 7) with 0.025% Proclin300 added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Pig, Rhesus Monkey(>80% identity)
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
• Publications:

  Androgen Receptor Is Dispensable for X-Zone Regression in the Female Adrenal but Regulates Post-Partum Corticosterone Levels and Protects Cortex Integrity. Front Endocrinol (Lausanne). 11, 599869 (2021). 33584538

  Androgen receptor signalling in the male adrenal facilitates X-zone regression, cell turnover and protects against adrenal degeneration during ageing. Sci Rep. 9, 10457 (2019). 31320667

Target Info

• Gene Symbol: AKR1C1
• Gene Full Name: Aldo-keto reductase family 1 member C1
• Alias Symbols: C9, DD1, DDH, DDH1, H-37, HBAB, MBAB, HAKRC, DD1/DD2, 2-ALPHA-HSD, 20-ALPHA-HSD
• NCBI Gene Id: 1645
• Protein Name: Aldo-keto reductase family 1 member C1
• Description of Target: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
• Uniprot ID: Q04828
• Protein Accession #: NP_001344.2
• Molecular Weight: 37 kDa