AKR1B10 Antibody (OAGA00776)

Catalog No: OAGA00776

Size:100 ul

Sample (30 ug of whole cell lysate)
A: MOLT4
B: Raji
10% SDS PAGE
OAGA00776 diluted at 1:1000

AKR1B10 antibody detects AKR1B10 protein by Western blot analysis.
A. 50 ug mouse colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (OAGA00776) dilution: 1:5000

AKR1B10 antibody detects AKR1B10 protein by Western blot analysis.
A. 50 ug rat colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (OAGA00776) dilution: 1:5000

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10(OAGA00776) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10(OAGA00776) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC, WB
• Additional Information: Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq]
  Positive Controls: Molt-4, Raji, mouse colon, rat colon
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 316 of AKR1B10 (Uniprot ID#O60218)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: AKR1B10
• Gene Full Name: Aldo-keto reductase family 1 member B10
• Alias Symbols: HIS, HSI, ARL1, ARL-1, ALDRLn, AKR1B11, AKR1B12
• NCBI Gene Id: 57016
• Protein Name: Aldo-keto reductase family 1 member B10
• Description of Target: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.
• Uniprot ID: O60218
• Protein Accession #: NP_064695.3
• Molecular Weight: 36 kDa