- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-AKAP12 antibody - OAGA00496 |
---|
Predicted Species Reactivity | Human |
---|---|
Product Format | Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB, ICC, IF, IHC-P |
Additional Information | Positive Controls: 293T , A431 , H1299 , HeLaS3 , HepG2 Cellular Localization: Cytoplasm, cell cortex, cytoskeleton |
Reconstitution and Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Predicted Homology Based on Immunogen Sequence | Rhesus Monkey (> 80% identity) |
Concentration | 0.73 mg/ml |
Application Info | WB: 1:500-1:3000 ICC: 1:100-1:1000 IF: 1:100-1:1000 IHC-P: 1:100-1:1000 Optimal dilutions should be determined by the end user. |
Gene Symbol | AKAP12 |
---|---|
Gene Full Name | A-kinase anchor protein 12 |
Alias Symbols | SSeCKS, AKAP250 |
NCBI Gene Id | 9590 |
Protein Name | A-kinase anchor protein 12 |
Description of Target | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
Uniprot ID | Q02952 |
Protein Accession # | NP_005091.2 |
Molecular Weight | 191 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "AKAP12 Antibody (OAGA00496)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
-
How long will it take to receive "AKAP12 Antibody (OAGA00496)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "AKAP12 Antibody (OAGA00496)" provided in?
This item is provided in "Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "AKAP12 Antibody (OAGA00496)"?
This target may also be called "SSeCKS, AKAP250" in publications.
-
What is the shipping cost for "AKAP12 Antibody (OAGA00496)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "AKAP12 Antibody (OAGA00496)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "AKAP12 Antibody (OAGA00496)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "191 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "AKAP12 Antibody (OAGA00496)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "AKAP12"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "AKAP12"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "AKAP12"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "AKAP12"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "AKAP12"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "AKAP12"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.