AKAP1 ELISA Kit (Human) (OKEI00133)

Catalog No: OKEI00133

Size:96 Wells

Human AKAP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology AKAP1 ELISA Kit (Human) (OKEI00133) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample AKAP1
• ELISA Kit Range: 0.625 - 40 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level AKAP1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level AKAP1 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • AKAP1 Microplate: 96 Wells (12 x 8 Well strips)
  • AKAP1 Lyophilized Standard: 2
  • 100X AKAP1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 0.375 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AKAP1
• Alias Symbols: AKAP, PRKA1, AKAP84, TDRD17, AKAP121, AKAP149, D-AKAP1, PPP1R43, SAKAP84
• NCBI Gene Id: 8165
• Protein Name: A-kinase anchor protein 1, mitochondrial
• Description of Target: Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
• Uniprot ID: Q92667
• Protein Accession #: NP_001229831
• Nucleotide Accession #: NM_001242902