AK3 ELISA Kit (Human) (OKCD00960)

Catalog No: OKCD00960 (Formerly GWB-KBBLT5)

Size:96WELLS

Human AK3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	92-101%	85-104%	97-105%	87-96%
  EDTA plasma(n=5)	98-105%	79-101%	94-101%	98-105%
  heparin plasma(n=5)	80-93%	86-101%	79-89%	81-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Kinase 3 (AK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Kinase 3 (AK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Kinase 3 (AK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenylate Kinase 3 (AK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-98	95
  EDTA plasma(n=5)	81-90	85
  heparin plasma(n=5)	95-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Kinase 3 (AK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Kinase 3 (AK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-AK3 Microplate: 96 Wells (12 x 8 Well Strips)
  • AK3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated AK3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cerebrospinal fluid and other biological fluids.
• Sensitivity: < 0.146 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Kinase 3 (AK3). No significant cross-reactivity or interference between Adenylate Kinase 3 (AK3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AK3
• Gene Full Name: adenylate kinase 3
• Alias Symbols: Adenylate kinase 3;adenylate kinase 3 alpha-like 1;adenylate kinase 6, adenylate kinase 3 like 1;AK3L1;AK6;AKL3L;AKL3L1;FIX;GTP:AMP phosphotransferase AK3, mitochondrial;GTP:AMP phosphotransferase, mitochondrial.
• NCBI Gene Id: 50808
• Protein Name: GTP:AMP phosphotransferase AK3, mitochondrial
• Description of Target: Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.
• Uniprot ID: Q9UIJ7
• Protein Accession #: NP_001186781
• Nucleotide Accession #: NM_001199852.1
• Protein Size (# AA): 227