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AIRE Antibody (OAEB02482)

100 ug
$349.00
In Stock
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Gene Symbol:
AIRE
NCBI Gene Id:
326
Protein Name:
Autoimmune regulator
Swissprot Id:
O43918
Protein Accession #:
NP_000374.1
Alias Symbols:
AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal
Replacement Item:
This antibody may replace item sc-17985 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express AIRE.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express AIRE.
Immunogen:
This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human: 100%
Complete computational species homology data:
AIRE Antibody (OAEB02482)
Peptide Sequence:
C-QSMARPAAPFPS
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-AIRE antibody - OAEB02482
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Appr. 60kDa in transfected HEK293 lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration, 2.5ug/ml.
Other Notes: ChIP: A customer has reported this antibody was successful in ChIP (no data).
Additional Information:
Reactivity Info: Tested: Human; Expected: Human
Target Reference:
* Ruan QG, Tung K, Eisenman D, Setiady Y, Eckenrode S, Yi B, Purohit S, Zheng WP, Zhang Y, Peltonen L, She JX.; The autoimmune regulator directly controls the expression of genes critical for thymic epithelial function.; J Immunol. 2007 Jun 1;178(11):7173-80.; Pubmed 17513766
* Kumar PG, Laloraya M, Wang CY, Ruan QG, Davoodi-Semiromi A, Kao KJ, She JX.; The autoimmune regulator (AIRE) is a DNA-binding protein.; J Biol Chem. 2001 Nov 2;276(44):41357-64. Epub 2001 Aug 31.; Pubmed 11533054

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