Catalog No: OKEH06848
Size:96T
Price: $525.00
SKU
OKEH06848
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology AIMP1 ELISA Kit (Human) (OKEH06848) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AIMP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AIMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AIMP1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into matrix = 92% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <6.5% Inter-Assay CV <7.8% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | AIMP1 |
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Gene Full Name | aminoacyl tRNA synthetase complex interacting multifunctional protein 1 |
Alias Symbols | aminoacyl tRNA synthase complex-interacting multifunctional protein 1;ARS-interacting multifunctional protein 1;EMAP2;EMAPII;endothelial monocyte-activating polypeptide 2;endothelial-monocyte activating polypeptide II;HLD3;multisynthase complex auxiliary component p43;multisynthetase complex auxiliary component p43;p43;SCYE1;small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating). |
NCBI Gene Id | 9255 |
Protein Name | Q12904 |
Description of Target | Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q12904 |
Protein Accession # | NP_001135887 |
Nucleotide Accession # | NM_001142415 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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