AIM1 ELISA Kit (Human) (OKCD00994)

Catalog No: OKCD00994 (Formerly GWB-KBBJM9)

Size:96WELLS

Human AIM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Absent In Melanoma 1 (AIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Absent In Melanoma 1 (AIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Absent In Melanoma 1 (AIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Absent In Melanoma 1 (AIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Absent In Melanoma 1 (AIM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Absent In Melanoma 1 (AIM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-AIM1 Microplate: 96 Wells (12 x 8 Well Strips)
  • AIM1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated AIM1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.134 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Absent In Melanoma 1 (AIM1). No significant cross-reactivity or interference between Absent In Melanoma 1 (AIM1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CRYBG1
• Gene Full Name: crystallin beta-gamma domain containing 1
• Alias Symbols: absent in melanoma 1 protein;AIM1;beta/gamma crystallin domain-containing protein 1;beta-gamma crystallin domain containing 1;ST4;suppression of tumorigenicity 4 (malignant melanoma).
• NCBI Gene Id: 202
• Protein Name: Q9Y4K1
• Description of Target: Beta/gamma crystallin domain-containing protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q9Y4K1
• Protein Accession #: NP_001615
• Nucleotide Accession #: NM_001624.3
• Protein Size (# AA): 1723