Catalog No: OKEH04429
Size:96T
Price: $525.00
SKU
OKEH04429
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Aifm1 ELISA Kit (Rat) (OKEH04429) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aifm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Aifm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aifm1 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.1% (n = 20)
Mean Inter-assay CV%: <= 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Aifm1 Microplate96 Wells (12 x 8 Well Strips)
Aifm1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Aifm1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat Apoptosis-inducing factor 1, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAifm1
Gene Full Nameapoptosis inducing factor, mitochondria associated 1
Alias SymbolsAif;apoptosis-inducing factor 1, mitochondrial;apoptosis-inducing factor 1, mitochondrial-like;apoptosis-inducing factor, mitochondrion-associated 1;Pdcd8;programmed cell death 8 (apoptosis-inducing factor);programmed cell death protein 8.
NCBI Gene Id83533
Protein NameQ9JM53
Description of TargetApoptosis-inducing factor 1, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/Q9JM53
Protein Accession #NP_112646.1
Nucleotide Accession #NM_031356.1
Protein Size (# AA)612
Molecular Weight67 KDa
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