Catalog No: OPPA02072 (Formerly GWB-5E6128)
Size:2UG
Price: $75.00
SKU
OPPA02072
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA02072 |
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Predicted Species Reactivity | Human |
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Product Format | Sterile filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5. |
Host | E. Coli |
Application | WB |
Additional Information | Solubility: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. |
:: | Product Introduction: Human AIF1 protein shares 98% homology/identity with that of rat. AIF1 is expressed in macrophages and neutrophils. The expression of AIF1 transcripts is upregulated by IFN-g in rat macrophages. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. In quiescent cultured human vascular smooth muscle cells synthesis of AIF1 is induced by IFN-g, IL1b, and conditioned medium of T-cells. Overexpression of AIF1 in human VSMCs results in enhanced growth of these cells. AIF1 is expressed during apoptosis rat mammary gland and ventral prostate tissues. Allograft Inflammatory Factor 1 is expressed by several tumor-associated activated macrophages and microglial cells in rat and human gliomas. There is an evident relationship of AIF1-expressing activated macrophages and microglial cells with tumor malignancy in humans. Product Description: The AIF1 Human Recombinant contains a total of 155 amino acids having a molecular Mass of 17.7kDa. The Human AIF1 is fused to a 9 amino acid long N-terminal FLAG tag. |
Reconstitution and Storage | For long term, store lyophilized AIF1 at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable for 24 months when stored at -20C. |
Purity | Greater than 90% as determined by SDS PAGE. |
Peptide Sequence | MKHHHHHHASQTRDLQGGKAF GLLKAQQEERLDE INKQFLDDPKYSSDED LPSKLEGFKEKYMEF DLNGNGDIDIMSLKRMLEK LGVPKTHLELKKLI GEVSSGSGETFSYP DFLRMMLGKRSAIL KMILMYEEKAREKEK PTGPPAKKAISELP. |
Gene Symbol | AIF1 |
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Alias Symbols | IBA1, IRT1, AIF-1, IRT-1 |
NCBI Gene Id | 199 |
Protein Name | Allograft inflammatory factor 1 |
Description of Target | Recombinant Human Allograft Inflammatory Factor 1 |
Uniprot ID | P55008 |
Protein Accession # | NP_001614.3 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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