Catalog No: OKCD08068
Size:96WELLS
Price: $825.00
SKU
OKCD08068
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Datasheets/ManualsPrintable datasheet for AIF1 ELISA Kit (Mouse) (OKCD08068)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ionized Calcium-binding Adapter Molecule 1 (IBA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ionized Calcium-binding Adapter Molecule 1 (IBA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AIF1 Microplate96 Wells (12 x 8 Well strips)
AIF1 Lyophilized Standard2
100X AIF1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity< 6.0pg/mL
Gene SymbolAif1
Gene Full Nameallograft inflammatory factor 1
Alias SymbolsAI607846;AIF-1;allograft inflammatory factor 1;D17H6S50;D17H6S50E;G1;Ib;Iba1;ionized calcium binding adapter molecule 1;Ionized calcium-binding adapter molecule 1;testis specific.
NCBI Gene Id11629
Protein NameAllograft inflammatory factor 1
Description of TargetActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Uniprot IDO70200
Protein Accession #NP_062340.1
Nucleotide Accession #NM_019467.2
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