AIF1 Chemi-Luminescent ELISA Kit (Human) (OKCD03291)

Catalog No: OKCD03291

Size:96WELLS

Human AIF1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ionized Calcium-binding Adapter Molecule 1 (IBA1) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AIF1 Microplate: 96 Wells (12 x 8 Well strips)
  • AIF1 Lyophilized Standard: 2
  • 100X AIF1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AIF1
• Gene Full Name: allograft inflammatory factor 1
• Alias Symbols: AIF-1;allograft inflammatory factor 1;IBA1;interferon gamma responsive transcript;ionized calcium-binding adapter molecule 1;IRT1;IRT-1;protein G1.
• NCBI Gene Id: 199
• Protein Name: Allograft inflammatory factor 1
• Description of Target: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
• Uniprot ID: P55008
• Protein Accession #: NP_001305899
• Nucleotide Accession #: NM_001318970