Catalog No: OKEH03111
Size:96T
Price: $525.00
SKU
OKEH03111
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AHSP ELISA Kit (Mouse) (OKEH03111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ahsp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ahsp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ahsp captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.8% (n = 20)
Mean Inter-assay CV%: <= 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Ahsp Microplate96 Wells (12 x 8 Well strips)
Ahsp Lyophilized Standard2
100X Biotinylated Ahsp Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Mouse Alpha-hemoglobin-stabilizing protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAhsp
Gene Full Namealpha hemoglobin stabilizing protein
Alias Symbolsalpha-hemoglobin-stabilizing protein;EDRF;Er;Eraf;erythroid differentiation-related factor;erythroid-associated factor.
NCBI Gene Id170812
Protein NameQ9CY02
Description of TargetAlpha-hemoglobin-stabilizing protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q9CY02
Protein Accession #NP_573508.1
Nucleotide Accession #NM_133245.1
Protein Size (# AA)475
Molecular Weight54 kDa
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