AHSP ELISA Kit (Human) (OKCD06320)

Catalog No: OKCD06320

Size:96WELLS

AHSP ELISA Kit (Human) (OKCD06320) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Hemoglobin Stabilizing Protein (aHSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Hemoglobin Stabilizing Protein (aHSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Hemoglobin Stabilizing Protein (aHSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-Hemoglobin Stabilizing Protein (aHSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Hemoglobin Stabilizing Protein (aHSP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Hemoglobin Stabilizing Protein (aHSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AHSP Microplate: 96 Wells (12 x 8 Well strips)
  • AHSP Lyophilized Standard: 2
  • 100X AHSP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 13.3pg/mL

Target Info

• Gene Symbol: AHSP
• Gene Full Name: alpha hemoglobin stabilizing protein
• Alias Symbols: alpha hemoglobin stabilising protein;alpha-hemoglobin-stabilizing protein;EDRF;ERAF;erythroid differentiation associated factor;erythroid differentiation-related factor;Erythroid-associated factor.
• NCBI Gene Id: 51327
• Protein Name: Alpha-hemoglobin-stabilizing protein
• Description of Target: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.
• Uniprot ID: Q9NZD4
• Protein Accession #: NP_001305150.1
• Nucleotide Accession #: NM_001318221.1