Catalog No: OKCD06108
Size:96WELLS
Price: $850.00
SKU
OKCD06108
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for AHSG ELISA Kit (Bovine) (OKCD06108)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Heremans Schmid Glycoprotein (AHSG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Heremans Schmid Glycoprotein (AHSG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Heremans Schmid Glycoprotein (AHSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-2-Heremans Schmid Glycoprotein (AHSG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Heremans Schmid Glycoprotein (AHSG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Heremans Schmid Glycoprotein (AHSG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AHSG Microplate96 Wells (12 x 8 Well strips)
AHSG Lyophilized Standard2
100X AHSG HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 2.45ng/mL
Gene SymbolAHSG
Gene Full Namealpha 2-HS glycoprotein
Alias Symbolsalpha-2-HS-glycoprotein;asialofetuin;fetuin-A.
NCBI Gene Id280988
Protein NameAlpha-2-HS-glycoprotein
Description of TargetPromotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
Uniprot IDP12763
Protein Accession #NP_776409.1
Nucleotide Accession #NM_173984.3
Write Your Own Review
You're reviewing:AHSG ELISA Kit (Bovine) (OKCD06108)
Your Rating
We found other products you might like!