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Catalog No: OKCD00574 (Formerly GWB-KBBZV3)
Size:96WELLS
Price: $675.00
SKU
OKCD00574
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Datasheets/ManualsPrintable datasheet for AHRR ELISA Kit (Human) (OKCD00574)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Repressor (AHRR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Repressor (AHRR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Repressor (AHRR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aryl Hydrocarbon Receptor Repressor (AHRR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Repressor (AHRR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Repressor (AHRR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-AHRR Microplate96 Wells (12 x 8 Well Strips)
AHRR Lyophilized Standard2 x 10 ng
100X Biotinylated AHRR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Repressor (AHRR). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Repressor (AHRR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAHRR
Gene Full Namearyl-hydrocarbon receptor repressor
Alias SymbolsAHH;AHHR;ahR repressor;aryl hydrocarbon hydroxylase regulator;aryl hydrocarbon receptor repressor;bHLHe77;class E basic helix-loop-helix protein 77;dioxin receptor repressor.
NCBI Gene Id57491
Protein NameAryl hydrocarbon receptor repressor
Description of TargetMediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.
Uniprot IDA9YTQ3
Protein Accession #NP_001229341
Nucleotide Accession #NM_001242412.1
Protein Size (# AA)701
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