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Catalog No: OKEH03344
Size:96T
Price: $525.00
SKU
OKEH03344
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AHR ELISA Kit (Mouse) (OKEH03344) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ahr has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ahr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ahr captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.6% (n = 20)
Mean Inter-assay CV%: <= 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Ahr Microplate96 Wells (12 x 8 Well strips)
Ahr Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ahr Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.084 ng/mL
SpecificityNatural and recombinant Mouse Aryl hydrocarbon receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAhr
Gene Full Namearyl-hydrocarbon receptor
Alias SymbolsA;Ah;ah receptor;Ahh;Ahre;aryl hydrocarbon receptor;bHLHe7;bHLHe76;dioxin receptor;In.
NCBI Gene Id11622
Protein NameP30561
Description of TargetAryl hydrocarbon receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P30561
Protein Accession #NP_038492.1
Nucleotide Accession #NM_013464.4
Protein Size (# AA)848
Molecular Weight95 kDa
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