AHNAK ELISA Kit (Human) (OKCD00575)

Catalog No: OKCD00575 (Formerly GWB-KBBQA5)

Size:96WELLS

Human AHNAK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoyokin (AHNAK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoyokin (AHNAK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoyokin (AHNAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Desmoyokin (AHNAK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmoyokin (AHNAK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmoyokin (AHNAK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-AHNAK Microplate: 96 Wells (12 x 8 Well Strips)
  • AHNAK Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated AHNAK Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Desmoyokin (AHNAK). No significant cross-reactivity or interference between Desmoyokin (AHNAK) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AHNAK
• Gene Full Name: AHNAK nucleoprotein
• Alias Symbols: AHNAK-related;AHNAKRS;desmoyokin;neuroblast differentiation-associated protein AHNAK;PM227.
• NCBI Gene Id: 79026
• Protein Name: Neuroblast differentiation-associated protein AHNAK
• Description of Target: May be required for neuronal cell differentiation.
• Uniprot ID: Q09666
• Protein Accession #: NP_001611
• Nucleotide Accession #: NM_001620.2
• Protein Size (# AA): 5890