Catalog No: OKEH03618
Price: $625.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03618
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AHCY ELISA Kit (Mouse) (OKEH03618) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ahcy has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ahcy is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ahcy captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 8.6% (n = 20)
ELISA Kit Component
Ahcy Microplate96 Wells (12 x 8 Well strips)
Ahcy Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ahcy Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.088 ng/mL
SpecificityNatural and recombinant Mouse Adenosylhomocysteinase

Obesity Prevents S-Adenosylmethionine-Mediated Improvements in Age-Related Peripheral and Hippocampal Outcomes. Nutrients. 13 (2021). 33917279

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAhcy, Ahcyl
Gene Full Nameadenosylhomocysteinase like, S-adenosylhomocysteine hydrolase
Alias SymbolsAA987153;adenosylhomocysteinase;adoHcyase;AL024110;CuB;CuBP;EG11615;Gm4737;liver copper-binding protein;SA;S-adenosyl-L-homocysteine hydrolase;SAHH.
NCBI Gene Id11615, 269378
Protein NameP50247
Description of TargetAdenosylhomocysteinase
Uniprot IDhttps://www.uniprot.org/uniprot/P50247
Protein Accession #NP_001291457.1
Nucleotide Accession #NM_001304528.1
Protein Size (# AA)432
Molecular Weight48 kDa
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