Aha1 Antibody : HRP (OASE00111-HRP)

Catalog No: OASE00111-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: 25F2.D10
• Isotype: IgG2a Kappa
• Host: Rat
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, ELISA, IP, IHC, ICC, IF
• Additional Information: Background Info: ~38kDa. Can run up to 45kDa on SDS Page
  Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Aha1 in 10ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Mouse Aha1
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
• Reference: 1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
  2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
  3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
  4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
  

Target Info

• Gene Symbol: Ahsa1
• Gene Full Name: AHA1, activator of heat shock protein ATPase 1
• Alias Symbols: Ah, p3, p38, BC023857
• NCBI Gene Id: 217737
• Uniprot ID: Q8BK64
• Protein Accession #: NP_666148.1