AGXT2 ELISA Kit (Human) (OKEH07616)

Catalog No: OKEH07616

Size:96T

Human AGXT2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology AGXT2 ELISA Kit (Human) (OKEH07616) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGXT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AGXT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGXT2 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=7.8% (n = 20)
• ELISA Kit Component:
  • AGXT2 Microplate: 96 Wells (12 x 8 Well strips)
  • AGXT2 Lyophilized Standard: 2
  • 100X Biotinylated AGXT2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.
  Subcellular Location: Mitochondrion
  Unigene: Hs.34494
  SMR: Q9BYV1
  String: 9606.ENSP00000231420
  KEGG: hsa:64902
  MIM: 612471
  Pfam: PF00202
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.22ng/mL
• Specificity: Natural and recombinant Human Alanine--glyoxylate aminotransferase 2, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AGXT2
• Gene Full Name: alanine--glyoxylate aminotransferase 2
• Alias Symbols: (R)-3-amino-2-methylpropionate--pyruvate transaminase;AGT2;alanine--glyoxylate aminotransferase 2, mitochondrial;BAIBA;beta-ALAAT II;beta-alanine-pyruvate aminotransferase;DAIBAT;D-AIBAT.
• NCBI Gene Id: 64902
• Protein Name: Q9BYV1
• Description of Target: Alanine--glyoxylate aminotransferase 2, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q9BYV1
• Protein Accession #: NP_001293102.1
• Nucleotide Accession #: NM_001306173.1