AGXT ELISA Kit (Mouse) (OKCA01771)

Catalog No: OKCA01771

Size:96 Wells

Mouse AGXT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology Agxt ELISA Kit (Mouse) (OKCA01771) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agxt has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Agxt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Agxt captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	93	89-97
  EDTA Plasma (n=4)	101	95-106

• ELISA Kit Component:
  • Agxt Microplate: 96 Wells (12 x 8 Well strips)
  • Agxt Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated Agxt Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: AGXT
• Gene Full Name: alanine-glyoxylate aminotransferase
• Alias Symbols: AGT, SPT, Agt1, Agxt1
• NCBI Gene Id: 11611
• Protein Name: Serine--pyruvate aminotransferase, mitochondrial
• Description of Target: Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).
• Uniprot ID: O35423
• Protein Accession #: NP_057911.2
• Nucleotide Accession #: NM_016702.3