AGTR1A ELISA Kit (Rat) (OKCD07561)

Catalog No: OKCD07561

Size:96WELLS

AGTR1A ELISA Kit (Rat) (OKCD07561) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II Receptor 1 (AGTR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin II Receptor 1 (AGTR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin II Receptor 1 (AGTR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin II Receptor 1 (AGTR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II Receptor 1 (AGTR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II Receptor 1 (AGTR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AGTR1A Microplate: 96 Wells (12 x 8 Well strips)
  • AGTR1A Lyophilized Standard: 2
  • 100X AGTR1A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: Agtr1a
• Gene Full Name: angiotensin II receptor, type 1a
• Alias Symbols: Agtr1;angiotensin II type-1A receptor;angiotensin receptor 1a;AT1;AT1A;AT1R;type-1A angiotensin II receptor;vascular type-1 angiotensin II receptor.
• NCBI Gene Id: 24180
• Protein Name: Type-1A angiotensin II receptor
• Description of Target: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
• Uniprot ID: P25095
• Protein Accession #: NP_112247.2
• Nucleotide Accession #: NM_030985.4