Catalog No: OKEH04682
Size:96T
Price: $525.00
SKU
OKEH04682
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Agtr1a ELISA Kit (Mouse) (OKEH04682) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agtr1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Agtr1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Agtr1a captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Agtr1a Microplate96 Wells (12 x 8 Well Strips)
Agtr1a Lyophilized Standard2
Sample Diluent1 x 20 mL
Agtr1a Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity42.5 pg/mL
SpecificityNatural and recombinant Mouse Type-1A angiotensin II receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAgtr1a
Gene Full Nameangiotensin II receptor, type 1a
Alias Symbols1810074K20Rik;AG2S;Agt;Agt1ar;Agtr;Agtr1;Agtr-1a;AI551199;angiotensin II type-1A receptor;angiotensin receptor 1;angiotensin receptor 1a;Angt;Angtr-1a;AT;AT1;AT1a;AT2R1;AT2R1A;type-1A angiotensin II receptor.
NCBI Gene Id11607
Protein NameP29754
Description of TargetType-1A angiotensin II receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P29754
Protein Accession #NP_796296.1
Nucleotide Accession #NM_177322.3
Protein Size (# AA)359
Molecular Weight41 KDa
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