Catalog No: OKEH04378
Size:96T
Price: $525.00
SKU
OKEH04378
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit LinearityMean recovery when spiking into serum: 95%
ELISA Kit PrincipleAviva Systems Biology Agt ELISA Kit (Rat) (OKEH04378) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agt has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Agt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Agt captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: <5.8% (n=20)
Mean Inter-Assay CV: <7.7% (n=24)
ELISA Kit Component
ComponentAmount
Anti-Agt Microplate96 Wells (12 x 8 Well Strips)
Agt Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Agt Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity8.06 pg/mL
SpecificityNatural and recombinant Rat Angiotensinogen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAgt
Gene Full Nameangiotensinogen
Alias SymbolsAng;AngII;angiotensin II;angiotensinogen;angiotensinogen (serpin peptidase inhibitor, clade A, member 8);ANRT;PAT;serpin A8.
NCBI Gene Id24179
Protein NameP01015
Description of TargetAngiotensinogen
Uniprot IDhttps://www.uniprot.org/uniprot/P01015
Protein Accession #NP_602308.1
Nucleotide Accession #NM_134432.2
Protein Size (# AA)477
Molecular Weight52 KDa
Write Your Own Review
You're reviewing:AGT ELISA Kit (Rat) (OKEH04378)
Your Rating
We found other products you might like!