AGT Chemi-Luminescent ELISA Kit (Human) (OKCD03287)

Catalog No: OKCD03287

Size:96WELLS

Human AGT ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-103%	88-98%	96-105%	94-104%
  EDTA Plasma (n=5)	92-103%	97-105%	95-102%	89-98%
  Heparin Plasma (n=5)	80-97%	91-98%	99-105%	84-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Angiotensinogen (AGT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensinogen (AGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensinogen (AGT) level in the sample or standard.
• ELISA Kit Range: 0.14-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-97	90
  EDTA Plasma (n=5)	84-98	90
  Heparin Plasma (n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AGT Microplate: 96 Wells (12 x 8 Well strips)
  • AGT Lyophilized Standard: 2
  • 100X AGT Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.56 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AGT
• Gene Full Name: angiotensinogen
• Alias Symbols: alpha-1 antiproteinase, antitrypsin;angiotensin I;angiotensin II;angiotensinogen;ANHU;fetal-liver predominant transporter 1;hFLT1;pre-angiotensinogen;serine (or cysteine) proteinase inhibitor;serpin A8;serpin peptidase inhibitor, clade A, member 8;SERPINA8.
• NCBI Gene Id: 183
• Protein Name: Angiotensinogen
• Description of Target: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
• Uniprot ID: P01019
• Protein Accession #: NP_000020
• Nucleotide Accession #: NM_000029