Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKEH06845
Size:96T
Price: $675.00
SKU
OKEH06845
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

AGRP ELISA Kit (Mouse) (OKEH06845)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Agrp ELISA Kit (Mouse) (OKEH06845) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agrp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Agrp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Agrp captured in the well.
ELISA Kit Range0.031-2ng/ml
ELISA Kit RecoveryMean recovery when spiking into matrix = 106.4%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <6.2%
Inter-Assay CV <7.7%
ELISA Kit Component
ComponentAmount
Agrp Microplate96 Wells (12 x 8 Well strips)
Agrp Lyophilized Standard2 x 2 ng
100X Agrp HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.0155 ng/mL
SpecificityNatural and recombinant Mouse Agouti-related protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAgrp
Gene Full Nameagouti related neuropeptide
Alias SymbolsAg;agouti-related protein;Agrt;Art;orexigenic neuropeptide.
NCBI Gene Id11604
Protein NameP56473
Description of TargetAgouti-related protein
Uniprot IDhttps://www.uniprot.org/uniprot/P56473
Protein Accession #NP_001258735
Nucleotide Accession #NM_001271806
Write Your Own Review
You're reviewing:AGRP ELISA Kit (Mouse) (OKEH06845)
Your Rating
We found other products you might like!