AGRP ELISA Kit (Mouse) : 96 Wells (OKCA00170)

Catalog No: OKCA00170

Size:96 Wells

Mouse AGRP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	94%	90-101%
  Serum (n=4)	1:2	95%	89-100%
  Serum (n=4)	1:4	93%	87-99%
  Serum (n=4)	1:8	94%	88-99%

• ELISA Kit Principle: Aviva Systems Biology AGRP ELISA Kit (Mouse) (OKCA00170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse AGRP has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mouse AGRP is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse AGRP captured in well.
• ELISA Kit Range: 15.6 pg/mL - 1,000 pg/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	91%	85-96%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	92%	85-97%

• ELISA Kit Reproducibility: Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
• ELISA Kit Component:
  • Anti-AGRP Microplate: 96 Well (12 x 8 Well Strips)
  • AGRP Standard (Lyophilized): 2 x 1 ng
  • 100X AGRP Detection Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 3.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse AGRP. No significant cross-reactivity or interference between mouse AGRP and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AGRP
• Gene Full Name: agouti related neuropeptide
• Alias Symbols: Ag, Art, Agrt
• NCBI Gene Id: 11604
• Protein Name: Agouti-related protein
• Description of Target: This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
• Uniprot ID: P56473
• Protein Accession #: NP_001258735
• Nucleotide Accession #: NM_001271806
• Protein Size (# AA): 131
• Molecular Weight: 14 kDa