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Catalog No: OKCA00170
Size:96 Wells
Price: $754.00
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AGRP ELISA Kit (Mouse) : 96 Wells (OKCA00170)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:194%90-101%
Serum (n=4)1:295%89-100%
Serum (n=4)1:493%87-99%
Serum (n=4)1:894%88-99%
ELISA Kit PrincipleAviva Systems Biology AGRP ELISA Kit (Mouse) (OKCA00170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse AGRP has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mouse AGRP is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse AGRP captured in well.
ELISA Kit Range15.6 pg/mL - 1,000 pg/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)91%85-96%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)92%85-97%
ELISA Kit ReproducibilityIntra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
ELISA Kit Component
Anti-AGRP Microplate96 Well (12 x 8 Well Strips)
AGRP Standard (Lyophilized)2 x 1 ng
100X AGRP Detection Antibody1 x 120 uL
100X HRP-Avidin1 x 120 uL
Detection Antibody Diluent1 x 15 mL
HRP-Avidin Diluent1 x 15 mL
Sample Diluent1 x 50 mL
25X Wash Buffer1 x 10 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 3.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse AGRP. No significant cross-reactivity or interference between mouse AGRP and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAGRP
Gene Full Nameagouti related neuropeptide
Alias SymbolsAg, Art, Agrt
NCBI Gene Id11604
Protein NameAgouti-related protein
Description of TargetThis gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
Uniprot IDP56473
Protein Accession #NP_001258735
Nucleotide Accession #NM_001271806
Protein Size (# AA)131
Molecular Weight14 kDa
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