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AGRP ELISA Kit (Human) : 96 Wells (OKEH02636)

96 Wells
$449.00
In Stock
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Predicted Species Reactivity:
Human
Application:
ELISA
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
AGRP
Official Gene Full Name:
agouti related neuropeptide
Alias Symbols:
Agouti-related protein, Agrt, AGRT, ART, ASIP2, MGC118963
NCBI Gene Id:
181
Protein Name:
Agouti-related protein
Description of Target:
This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
Swissprot Id:
O00253
Protein Accession #:
NP_001129.1
Nucleotide Accession #:
NM_001138.1
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
10 pg/mL
Kit Range:
15.6 - 1000pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.9% (n = 20)
Mean Inter-assay CV%: < 11.7% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology AGRP ELISA Kit (Human) (OKEH02636) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGRP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for AGRP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGRP captured in well.
Kit Component:
ComponentAmount
Anti-AGRP Microplate96 Wells (12 x 8 Well strips)
AGRP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated AGRP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express AGRP.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express AGRP.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Agouti-related protein
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
14 kDa
Protein Size (# AA):
132

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