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Catalog No: OKEH02636
Price: $425.00
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AGRP ELISA Kit (Human) : 96 Wells (OKEH02636)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AGRP ELISA Kit (Human) (OKEH02636) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGRP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for AGRP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGRP captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.9% (n = 20)
Mean Inter-assay CV%: < 11.7% (n = 20)
ELISA Kit Component
Anti-AGRP Microplate96 Wells (12 x 8 Well strips)
AGRP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated AGRP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Agouti-related protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAGRP
Gene Full Nameagouti related neuropeptide
Alias Symbolsagouti related protein homolog;agouti-related protein;AGRT;ART;ASIP2.
NCBI Gene Id181
Protein NameO00253
Description of TargetAgouti-related protein
Uniprot ID
Protein Accession #NP_001129.1
Nucleotide Accession #NM_001138.1
Protein Size (# AA)132
Molecular Weight14 kDa
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