AGR3 Antibody - C-terminal region (OAAB06017)

Catalog No: OAAB06017

Size:400UL

Western blot analysis of AGR3 Antibody (C-term) in MCF-7 cell lysates (35ug/lane). AGR3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with AGR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AGR3 Antibody (C-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AGR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the C-terminal region of human AGR3.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: AGR3 Antibody (C-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: AGR3
• Alias Symbols: AG-3, AG3, Anterior gradient 3 homolog, anterior gradient homolog 3, anterior gradient protein 3, anterior gradient protein 3 homolog, BCMP11, breast cancer membrane protein 11, hAG-3, HAG3, PDIA18, protein disulfide isomerase family A, member 18.
• NCBI Gene Id: 155465
• Protein Name: Anterior gradient protein 3|anterior gradient protein 3 precursor [Homo sapiens]
• Description of Target: Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.
• Uniprot ID: Q8TD06
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_789783.1
• Molecular Weight: 19171