AGR2 ELISA Kit (Human) (OKCD01656)

Catalog No: OKCD01656 (Formerly GWB-KBBDU1)

Size:96WELLS

Human AGR2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	80-99%	84-93%	94-105%	98-105%
  EDTA Plasma (n=5)	90-99%	92-104%	96-105%	80-103%
  Heparin Plasma (n=5)	83-104%	80-89%	78-90%	94-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient 2 (AGR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anterior Gradient 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anterior Gradient 2 (AGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anterior Gradient 2 (AGR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-96	89
  EDTA Plasma (n=5)	86-102	98
  Heparin Plasma (n=5)	86-95	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-AGR2 Microplate: 96 Wells (12 x 8 Well Strips)
  • AGR2 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated AGR2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.62 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anterior Gradient Protein 2 (AGR2). No significant cross-reactivity or interference between Anterior Gradient Protein 2 (AGR2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AGR2
• Gene Full Name: anterior gradient 2, protein disulphide isomerase family member
• Alias Symbols: AG2;AG-2;anterior gradient homolog 2;anterior gradient protein 2 homolog;epididymis secretory protein Li 116;GOB-4;HAG-2;HEL-S-116;HPC8;PDIA17;protein disulfide isomerase family A, member 17;secreted cement gland homolog;secreted cement gland protein XAG-2 homolog;XAG-2.
• NCBI Gene Id: 10551
• Protein Name: Anterior gradient protein 2 homolog
• Description of Target: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).
• Uniprot ID: O95994
• Protein Accession #: NP_006399.1
• Nucleotide Accession #: NM_006408.3
• Protein Size (# AA): 175