AGR2 Chemi-Luminescent ELISA Kit (Human) (OKCD03286)

Catalog No: OKCD03286

Size:96WELLS

Human AGR2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-99%	89-97%	79-104%	99-105%
  EDTA Plasma (n=5)	78-93%	97-104%	85-97%	99-105%
  Heparin Plasma (n=5)	91-98%	79-105%	98-105%	95-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient 2 (AGR2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Anterior Gradient 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Anterior Gradient 2 (AGR2) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	99-105	102
  EDTA Plasma (n=5)	78-98	84
  Heparin Plasma (n=5)	93-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AGR2 Microplate: 96 Wells (12 x 8 Well strips)
  • AGR2 Lyophilized Standard: 2
  • 100X AGR2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0137 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AGR2
• Gene Full Name: anterior gradient 2, protein disulphide isomerase family member
• Alias Symbols: AG2;AG-2;anterior gradient homolog 2;anterior gradient protein 2 homolog;epididymis secretory protein Li 116;GOB-4;HAG-2;HEL-S-116;HPC8;PDIA17;protein disulfide isomerase family A, member 17;secreted cement gland homolog;secreted cement gland protein XAG-2 homolog;XAG-2.
• NCBI Gene Id: 10551
• Protein Name: Anterior gradient protein 2 homolog
• Description of Target: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).
• Uniprot ID: O95994
• Protein Accession #: NP_006399
• Nucleotide Accession #: NM_006408