AGP ELISA Kit (Feline) (OKIA00195)

Catalog No: OKIA00195

Size:1 x 96 well plate

Representative Feline AGP Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Feline
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 100 Minutes
• ELISA Kit Principle: The Aviva Systems Biology AGP ELISA Kit (Feline) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline Alpha1-glycoprotein acid in Plasma or Serum. This assay employs an antibody specific for Feline AGP coated on a 96-well plate. Standards and samples are pipetted into the wells and Alpha1-glycoprotein acid present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-AGP antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AGP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of AGP in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 1.57 - 100 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Feline AGP Microplate: 96 Wells (12 x 8 Well Strips)
  • Feline AGP Calibrator: 1
  • 100X AGP-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The AGP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP/Alpha-1-glycoprotein acid in Feline biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 0.8 ng/mL

Target Info

• Alias Symbols: AGP;agp;alpha1-gycoprotein acid;
• Protein Name: Alpha1-glycoprotein acid
• Description of Target: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction (By similarity).
• Uniprot ID: Q6KCA0