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Catalog No: OKEH05654
Size:96T
Price: $625.00
SKU
OKEH05654
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH05654
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ago2 ELISA Kit (Mouse) (OKEH05654) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ago2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ago2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ago2 captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 81.2%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <7.9%, n = 20
Mean Inter-Assay Precision - <10.1%, n = 20
ELISA Kit Component
ComponentAmount
AGO2 Microplate96 Wells (12 x 8 Well strips)
AGO2 Lyophilized Standard2
100X Biotinylated AGO2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.68 pg/mL
SpecificityNatural and recombinant Mouse Protein argonaute-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAgo2
Gene Full Nameargonaute RISC catalytic subunit 2
Alias Symbols1110029L17Rik;2310051F07Rik;AI225898;AL022874;argonaute 2;argonaute RISC catalytic component 2;AW546247;Eif2c;Eif2c2;Eukaryotic translation initiation factor 2C 2;eukaryotic translation initiation factor 2C, 2;Gerp95;Gm10365;mKIAA4215;Piwi/Argonaute family protein meIF2C2;protein argonaute-2;Protein slicer.
NCBI Gene Id239528
Protein NameQ8CJG0
Description of TargetProtein argonaute-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q8CJG0
Protein Accession #NP_694818.3
Nucleotide Accession #NM_153178.4
Protein Size (# AA)860
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