AGO2 Antibody (OACA02418)

Catalog No: OACA02418

Size:100ug

All lanes: Protein argonaute-2 antibody at 2 ug/ml Lane 1: A549 whole cell lysate Lane 2: Jurkats whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Raw264.7 whole cell lysate Lane 6: K562 whole cell lysate Lane 7: Mouse liver tissue Lane 8: Mouse kidney tissue Goat polyclonal to Rabbit IgG at 1/10,000 dilution Predicted band size: 97 kDa Observed band size: 90 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using OACA02418 at dilution 1: 100

Immunohistochemistry of paraffin-embedded human breast cancer using OACA02418 at dilution 1: 100

Immunohistochemistry of paraffin-embedded human gastric cancer using OACA02418 at dilution 1: 100

Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the SLC1A5 promoter.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Protein argonaute-2 protein (517-818AA)
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Specificity: Human
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Reference: [1] The crystal structure of human Argonaute2. Schirle N.T., MacRae I.J. Science 336:1037-1040 (2012).
  [2] The structure of human argonaute-2 in complex with miR-20a. Elkayam E., Kuhn C.D., Tocilj A., Haase A.D., Greene E.M., Hannon G.J., Joshua-Tor L. Cell 150:100-110 (2012).
  [3] Structural analysis of 5\'-mRNA-cap interactions with the human AGO2 MID domain. Frank F., Fabian M.R., Stepinski J., Jemielity J., Darzynkiewicz E., Sonenberg N., Nagar B. EMBO Rep. 12:415-420 (2011).

Target Info

• Gene Symbol: AGO2
• Gene Full Name: argonaute RISC catalytic component 2
• Alias Symbols: argonaute 2, RISC catalytic component;Argonaute RISC catalytic component 2;cancer susceptibility candidate 7 (non-protein coding);CASC7;EIF2C2;Eukaryotic translation initiation factor 2C 2;eukaryotic translation initiation factor 2C, 2;LESKRES;LINC00980;long intergenic non-protein coding RNA 980;PAZ Piwi domain protein;PPD;protein argonaute-2;protein slicer;Q10.
• NCBI Gene Id: 27161
• Protein Name: Protein argonaute-2
• Description of Target: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
• Uniprot ID: Q9UKV8
• Protein Accession #: NP_001158095.1
• Nucleotide Accession #: NM_001164623.1
• Protein Size (# AA): 859
• Molecular Weight: 97 kDa