Catalog No: OKCD01220 (Formerly GWB-KBBPA4)
Size:96WELLS
Price: $750.00
SKU
OKCD01220
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Datasheets/ManualsPrintable datasheet for AGGF1 ELISA Kit (Mouse) (OKCD01220)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-98%91-105%83-92%96-104%
EDTA plasma(n=5)95-102%97-104%99-105%97-105%
heparin plasma(n=5)78-103%99-105%90-97%85-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-104101
EDTA plasma(n=5)89-9793
heparin plasma(n=5)80-10186
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Aggf1 Microplate96 Wells (12 x 8 Well Strips)
Aggf1 Lyophilized Standard2 x 5 ng
100X Biotinylated Aggf1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity< 34 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). No significant cross-reactivity or interference between Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAggf1
Gene Full Nameangiogenic factor with G patch and FHA domains 1
Alias Symbols2010009L17Rik;2310029P06Rik;angiogenic factor VG5Q;angiogenic factor with G patch and FHA domains 1;AW112072;Peg3;VG5;VG5Q.
NCBI Gene Id66549
Protein NameAngiogenic factor with G patch and FHA domains 1
Description of TargetPromotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion (By similarity).
Uniprot IDQ7TN31
Protein Accession #NP_079906
Nucleotide Accession #NM_025630.3
Protein Size (# AA)711
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