AGER Chemi-Luminescent ELISA Kit (Mouse) (OKCD03285)

Catalog No: OKCD03285

Size:96WELLS

Mouse AGER ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-102%	93-105%	78-105%	81-97%
  EDTA Plasma (n=5)	78-93%	90-101%	98-105%	95-102%
  Heparin Plasma (n=5)	94-103%	95-102%	82-98%	95-102%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Receptor For Advanced Glycation Endproducts (RAGE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Receptor For Advanced Glycation Endproducts (RAGE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Receptor For Advanced Glycation Endproducts (RAGE) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-92	87
  EDTA Plasma (n=5)	84-101	95
  Heparin Plasma (n=5)	80-93	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor For Advanced Glycation Endproducts (RAGE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor For Advanced Glycation Endproducts (RAGE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Ager Microplate: 96 Wells (12 x 8 Well strips)
  • Ager Lyophilized Standard: 2
  • 100X Ager Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0137 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ager
• Gene Full Name: advanced glycosylation end product-specific receptor
• Alias Symbols: advanced glycation end-products receptor;advanced glycosylation end product-specific receptor;RA;RAGE;receptor for advanced glycation end-products variant 20;receptor for advanced glycosylation end products.
• NCBI Gene Id: 11596
• Protein Name: Advanced glycosylation end product-specific receptor
• Description of Target: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease.
• Uniprot ID: Q62151
• Protein Accession #: NP_001258351
• Nucleotide Accession #: NM_001271422