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Catalog No: OKEH07335
Size:96T
Price: $600.00
SKU
OKEH07335
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)85-85% (n = 20)86-95% (n = 20)112-122% (n = 20)95-105% (n = 20)
EDTA plasma(n=5)98-109% (n = 20)94-103% (n = 20)95-103% (n = 20)99-109% (n = 20)
heparin plasma(n=5)96-108% (n = 20)84-94% (n = 20)100-110% (n = 20)105-116% (n = 20)
ELISA Kit PrincipleAviva Systems Biology AFP ELISA Kit (Dog) (OKEH07335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AFP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AFP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AFP captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.2% (n = 20)
Mean Inter-assay CV%: <=6.2% (n = 20)
ELISA Kit Component
ComponentAmount
AFP Microplate96 Wells (12 x 8 Well strips)
AFP Lyophilized Standard2
100X Biotinylated AFP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
::Subcellular Location: Secreted
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.15ng/mL
SpecificityNatural and recombinant Dog Alpha-fetoprotein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAFP
Gene Full Namealpha fetoprotein
Alias Symbolsalpha-1-fetoprotein;alpha-fetoglobulin;alpha-fetoprotein.
NCBI Gene Id403551
Protein NameQ8MJU5
Description of TargetAlpha-fetoprotein
Uniprot IDhttps://www.uniprot.org/uniprot/Q8MJU5
Protein Accession #NP_001003027.1
Nucleotide Accession #NM_001003027.1
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