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Aflatoxin Total ELISA Kit (OKAO00139)

96 Wells
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Description of Target:
Aflatoxins are poisonous and cancer-causing chemicals that are produced by certain molds (Aspergillus flavus and Aspergillus parasiticus) which grow in soil, decaying vegetation, hay, and grains. They are regularly found in improperly stored staple commodities such as cassava, chili peppers, corn, cotton seed, millet, peanuts, rice, sorghum, sunflower seeds, tree nuts, wheat, and a variety of spices. When contaminated food is processed, aflatoxins enter the general food supply where they have been found in both pet and human foods, as well as in feedstocks for agricultural animals. Animals fed contaminated food can pass aflatoxin transformation products into eggs, milk products, and meat.
Protein Name:
Total Aflatoxins
Sample Type:
Cereal, compound feed, cooking oil, peanut, sauce, wheat and other feed, beer, wine, soy sauce and vinegar.
Cereal0.1 ppb
Compound feed0.2 ppb
Cooking oil, peanut0.2 ppb
Sauce, wheat and other feed0.2 ppb
Beer0.2 ppb
Wine, soy sauce, vinegar0.1 ppb
Kit Range:
0.02-1.62 ppb
Kit Reproducibility:
Mean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology Aflatoxin Total ELISA Kit (OKAO00139) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Aflatoxin Total has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Aflatoxin Total antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
Kit Component:
Aflatoxin Total Microplate96 Wells (12 x 8 Well Strips)
Aflatoxin Total Standards6 x 1 mL
Aflatoxin Total Rabbit Detector Antibody1 x 6 mL
Anti-Rabbit / HRP Conjugate1 x 6 mL
Detection Reagent A1 x 6 mL
Detection Reagent B1 x 6 mL
Stop Solution1 x 6 mL
20X Wash Buffer1 x 20 mL
Kit Recovery:
Cereal and compound feed85% +/- 15%
Peanut82% +/- 15%
Cooking oil85% +/- 15%
Sauce, wheat and other feed83% +/- 15%
Beer84% +/- 15%
Wine, soy sauce, vinegar87% +/- 15%
Kit Detection Method:
Colorimetric, OD450 nm
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
SubstanceCross Reactivity Rate
Aflatoxin B1100%
Aflatoxin B275%
Aflatoxin G1100%
Aflatoxin G297%
Aflatoxin M130%
Assay Info:
Assay Methodology: Competitive Inhibition ELISA

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