Aflatoxin Total ELISA Kit (OKAO00139)

Catalog No: OKAO00139

Size:96 Wells

Product Info

• Predicted Species Reactivity: General
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Aflatoxin Total ELISA Kit (OKAO00139) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Aflatoxin Total has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Aflatoxin Total antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
• ELISA Kit Range: 0.02-1.62 ppb
• ELISA Kit Recovery:

  Sample	Amount
  Cereal and compound feed	85% +/- 15%
  Peanut	82% +/- 15%
  Cooking oil	85% +/- 15%
  Sauce, wheat and other feed	83% +/- 15%
  Beer	84% +/- 15%
  Wine, soy sauce, vinegar	87% +/- 15%

• ELISA Kit Reproducibility: Mean Intra-Assay: CV<8% (n= 3 x 20)
  Mean Inter-Assay: CV<12% (n= 3 x 40)
• ELISA Kit Component:
  • Aflatoxin Total Microplate: 96 Wells (12 x 8 Well Strips)
  • Aflatoxin Total Standards: 6 x 1 mL
  • Aflatoxin Total Rabbit Detector Antibody: 1 x 6 mL
  • Anti-Rabbit / HRP Conjugate: 1 x 6 mL
  • Detection Reagent A: 1 x 6 mL
  • Detection Reagent B: 1 x 6 mL
  • Stop Solution: 1 x 6 mL
  • 20X Wash Buffer: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Cereal, compound feed, cooking oil, peanut, sauce, wheat and other feed, beer, wine, soy sauce and vinegar.
• Sensitivity:

  Sample	Amount
  Cereal	0.1 ppb
  Compound feed	0.2 ppb
  Cooking oil, peanut	0.2 ppb
  Sauce, wheat and other feed	0.2 ppb
  Beer	0.2 ppb
  Wine, soy sauce, vinegar	0.1 ppb

• Specificity:

  Substance	Cross Reactivity Rate
  Aflatoxin B1	100%
  Aflatoxin B2	75%
  Aflatoxin G1	100%
  Aflatoxin G2	97%
  Aflatoxin M1	30%

• Assay Info: Assay Methodology: Competitive Inhibition ELISA

Target Info

• Alias Symbols: AFT
• Protein Name: Total Aflatoxins
• Description of Target: Aflatoxins are poisonous and cancer-causing chemicals that are produced by certain molds (Aspergillus flavus and Aspergillus parasiticus) which grow in soil, decaying vegetation, hay, and grains. They are regularly found in improperly stored staple commodities such as cassava, chili peppers, corn, cotton seed, millet, peanuts, rice, sorghum, sunflower seeds, tree nuts, wheat, and a variety of spices. When contaminated food is processed, aflatoxins enter the general food supply where they have been found in both pet and human foods, as well as in feedstocks for agricultural animals. Animals fed contaminated food can pass aflatoxin transformation products into eggs, milk products, and meat.