Catalog No: OKCD00586 (Formerly GWB-KBBIW4)
Size:96WELLS
Price: $675.00
SKU
OKCD00586
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Datasheets/ManualsPrintable datasheet for AFF1 ELISA Kit (Human) (OKCD00586)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AF4/FMR2 Family, Member 1 (AFF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AF4/FMR2 Family, Member 1 (AFF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AF4/FMR2 Family, Member 1 (AFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AF4/FMR2 Family, Member 1 (AFF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-AFF1 Microplate96 Wells (12 x 8 Well Strips)
AFF1 Lyophilized Standard2 x 10 ng
100X Biotinylated AFF1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of AF4/FMR2 Family, Member 1 (AFF1). No significant cross-reactivity or interference between AF4/FMR2 Family, Member 1 (AFF1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAFF1
Gene Full NameAF4/FMR2 family member 1
Alias SymbolsAF4;AF4/FMR2 family member 1;ALL1-fused gene from chromosome 4 protein;MLLT2;myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2;PBM1;pre-B-cell monocytic leukemia partner 1;Protein FEL;proto-oncogene AF4.
NCBI Gene Id4299
Protein NameAF4/FMR2 family member 1
Description of TargetAF4/FMR2 family member 1
Uniprot IDP51825
Protein Accession #NP_001160165
Nucleotide Accession #NM_001166693.2
Protein Size (# AA)1210
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