AES Antibody (OAPB00345)

Catalog No: OAPB00345

Size:100UG

Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 μg/mL.

Immunofluorescence of AES in Hela cells with AES antibody at 20 ug/mL.

Immunocytochemistry of AES in HeLa cells with AES antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. AES Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Additional Information: Product Description: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: AES antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. The immunogen is located within the last 50 amino acids of AES.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-AES (OAPB00345) antibody is Catalog # OEPB02435
• Application Info: AES antibody can be used for the detection of AES by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.;Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.;Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.;Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.

Target Info

• Gene Symbol: TLE5
• Gene Full Name: TLE family member 5, transcriptional modulator
• Alias Symbols: AES;AES-1;AES-2;amino-terminal enhancer of split;ESP1;gp130-associated protein GAM;GRG;Grg-5;GRG5;groucho-related protein 5;Protein ESP1;Protein GRG;TLE family member 5.
• NCBI Gene Id: 166
• Protein Name: Amino-terminal enhancer of split|amino-terminal enhancer of split isoform a [Homo sapiens]
• Description of Target: Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
• Uniprot ID: Q08117
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_945320
• Nucleotide Accession #: NM_001130.5; NM_198969.1; NM_198970.1