AEN Antibody (OAGA00945)

Catalog No: OAGA00945

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
OAGA00945 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Apoptosis-enhancing nuclease(OAGA00945) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis.
  Positive Controls: HeLa, HepG2, Molt-4
  Cellular Localization: Nucleus, nucleolus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 236 and 325 of Apoptosis-enhancing nuclease (Uniprot ID#Q8WTP8)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.9 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: AEN
• Gene Full Name: Apoptosis-enhancing nuclease
• Alias Symbols: ISG20L1, pp12744
• NCBI Gene Id: 64782
• Protein Name: Apoptosis-enhancing nuclease
• Uniprot ID: Q8WTP8
• Protein Accession #: NP_073604.3
• Molecular Weight: 36 kDa