AEBP1 Antibody - N-terminal region (ARP31592_P050)

Catalog No: ARP31592_P050

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

Human Spermatophore

Human Intestine

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AEBP1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: VPPEKTKDKGKKGKKDKGPKVPKESLEGSPRPPKKGKEKPPKATKKPKEK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-AEBP1 (ARP31592_P050) antibody is Catalog # AAP31592 (Previous Catalog # AAPP02373)
• Enhanced Validation:

  Relative Expression (Western Blot)
  SPR Affinity Characterization

• Publications:

  Kim, H. J. et al. Identification of estradiol/ERa-regulated genes in the mouse pituitary. J. Endocrinol. 210, 309-21 (2011). 21700660

Target Info

• Gene Symbol: AEBP1
• Gene Full Name: AE binding protein 1
• Alias Symbols: ACLP
• NCBI Gene Id: 165
• Protein Name: Adipocyte enhancer-binding protein 1
• Description of Target: The adipocyte enhancer binding protein 1 (AEBP1) is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. AEBP1 may play a role in differentiated vascular smooth muscle cells.
• Uniprot ID: Q14113
• Protein Accession #: NP_001120
• Nucleotide Accession #: NM_001129
• Protein Size (# AA): 1158
• Molecular Weight: 131 kDa
• Protein Interactions: GRB2; TCF3;