Adrenaline ELISA Kit (Human) (OKRF00001)

Catalog No: OKRF00001

Size:96 Wells

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Duration: Extraction and acylation 65 minutes and ELISA 3 hours.
• ELISA Kit Principle: Enzyme Immunoassay for the quantitative determination of Adrenaline (Epinephrine) in plasma and urine. For in-vitro diagnostic use only. Adrenaline (epinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in quilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
• ELISA Kit Range: 0/1 - 200 ng/ml
• ELISA Kit Component:
  • Adhesive Foil: 1 x 4
  • Wash Buffer Concentrate: 1 x 20 mL
  • Enzyme Conjugate: 1 x 12 mL
  • Substrate: 1 x 12 mL
  • Stop Solution: 1 x 12 mL
  • Adrenaline Metanephrine Microtiter Strips: 1 x 96 wells
  • Adrenaline Antiserum: 1 x 6 mL
  • Adjustment Buffer: 1 x 4 mL
  • Standard A: 1 x 4 mL
  • Standard B: 1 x 4 mL
  • Standard C: 1 x 4 mL
  • Standard D: 1 x 4 mL
  • Standard E: 1 x 4 mL
  • Standard F: 1 x 4 mL
  • Acylation Buffer: 1 x 20 mL
  • Acylation Reagent: 1 x 3 ml
  • Assay Buffer: 1 x 6 mL
  • Coenzyme: 1 x 2 mL
  • Enzyme: 2 x 1 mL
  • Extraction Buffer: 1 x 6 mL
  • Extraction Plate: 2 x 48 wells
  • Hydrochloric Acid: 1 x 30 mL
  • Control 1: 1 x 4 mL
  • Control 2: 1 x 4 mL
• Additional Information: Design: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use.
  Advantages: Total assay approximately 2 h 15 min.
  No known interference by medical drugs.
  Wide standard ranges. convenient measurement of pathological samples without predilution.
  High correlation with HPLC.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: 10 ul urine, 300 ul plasma
• Sensitivity: 0.3 ng/ml urine, 10 pg/ml plasma

Target Info

• Gene Symbol: Adrenaline