ADRBK1 ELISA Kit (Human) (OKCD01648)

Catalog No: OKCD01648 (Formerly GWB-KBBEO4)

Size:96WELLS

Human ADRBK1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	98-105%	88-104%	86-101%	86-101%
  EDTA Plasma (n=5)	78-102%	91-104%	90-97%	95-105%
  Heparin Plasma (n=5)	79-93%	85-102%	99-105%	84-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta Adrenergic Receptor Kinase (BARK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta Adrenergic Receptor Kinase (BARK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta Adrenergic Receptor Kinase (BARK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Beta Adrenergic Receptor Kinase (BARK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	84-105	101
  EDTA Plasma (n=5)	79-91	85
  Heparin Plasma (n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta Adrenergic Receptor Kinase (BARK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta Adrenergic Receptor Kinase (BARK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADRBK1 Microplate: 96 Wells (12 x 8 Well Strips)
  • ADRBK1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ADRBK1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Beta Kinase 1 (ADRbK1). No significant cross-reactivity or interference between Adrenergic Receptor Beta Kinase 1 (ADRbK1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GRK2
• Gene Full Name: G protein-coupled receptor kinase 2
• Alias Symbols: ADRBK1;adrenergic beta receptor kinase 1;BARK1;beta-adrenergic receptor kinase 1;beta-ARK-1;BETA-ARK1;G-protein coupled receptor kinase 2.
• NCBI Gene Id: 156
• Protein Name: Beta-adrenergic receptor kinase 1
• Description of Target: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed:19306925, PubMed:19715378). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).
• Uniprot ID: P25098
• Protein Accession #: NP_001610.2
• Nucleotide Accession #: NM_001619.3
• Protein Size (# AA): 689