Adra1a ELISA Kit (Rat) (OKEH04818)

Catalog No: OKEH04818

Size:96T

Rat Adra1a ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Adra1a ELISA Kit (Rat) (OKEH04818) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adra1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adra1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adra1a captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Adra1a Microplate: 96 Wells (12 x 8 Well Strips)
  • Adra1a Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Adra1a Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.087 ng/mL
• Specificity: Natural and recombinant Rat Alpha-1A adrenergic receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Adra1a
• Gene Full Name: adrenoceptor alpha 1A
• Alias Symbols: Adra1c;adrenergic alpha 1c receptor;adrenergic receptor alpha 1c subtype;adrenergic receptor, alpha 1a;adrenergic receptor, alpha 1c;adrenergic, alpha-1A-, receptor;alpha-1A adrenergic receptor;alpha-1A adrenoceptor;alpha-1A adrenoreceptor;alpha-1C adrenergic receptor.
• NCBI Gene Id: 29412
• Protein Name: P43140
• Description of Target: Alpha-1A adrenergic receptor
• Uniprot ID: https://www.uniprot.org/uniprot/P43140
• Protein Accession #: NP_058887.2
• Nucleotide Accession #: NM_017191.2
• Protein Size (# AA): 466
• Molecular Weight: 52 kDa