ADORA2A ELISA Kit (Human) (OKCD08879)

Catalog No: OKCD08879

Size:96WELLS

ADORA2A ELISA Kit (Human) (OKCD08879) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine A2a Receptor (ADORA2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine A2a Receptor (ADORA2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine A2a Receptor (ADORA2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenosine A2a Receptor (ADORA2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine A2a Receptor (ADORA2a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine A2a Receptor (ADORA2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ADORA2A Microplate: 96 Wells (12 x 8 Well strips)
  • ADORA2A Lyophilized Standard: 2
  • 100X ADORA2A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.060ng/mL

Target Info

• Gene Symbol: ADORA2A
• Gene Full Name: adenosine A2a receptor
• Alias Symbols: A2aR;adenosine receptor A2a;adenosine receptor subtype A2a;ADORA2;RDC8.
• NCBI Gene Id: 135
• Protein Name: Adenosine receptor A2a
• Description of Target: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
• Uniprot ID: P29274
• Protein Accession #: NP_000666.2
• Nucleotide Accession #: NM_000675.5