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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for OALA02443 |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Rabbit, Mouse, Dog, Guinea pig, Rat, Human |
| Product Format | Liquid0.01M Tris-Glycine (pH 7.4), 0.15M sodium chloride, 0.05% sodium azide |
| Clonality | Monoclonal |
| Clone | 7F6-G5-A2 / 7F6 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | FC, IHC, IHC-P, IHC-PFA, WB |
| Additional Information | Antigen Type: Recombinant Protein |
| Reconstitution and Storage | Short term: store at 4C. Long term: aliquot and store at -20C. Avoid freeze-thaw cycles. |
| Immunogen | 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. |
| Purification | Protein G purified |
| Concentration | 0.5 mg/ml |
| Specificity | Recognizes human adenosine receptor A2A at ~43kD. |
| Epitope | 3rd Intracellular Domain |
| Warning | For research use only. |
| Application Info | FC, IHC, IHC-P (5 ug/ml), IHC-PFA (1 ug/ml), WB (1:1000) |
| Application Data | Immunohistochemistry: OALA02443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02443 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras. |
| Storage Buffer | 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide |
| Publications | Toll-like receptor 2 induces adenosine receptor A2a and promotes human squamous carcinoma cell growth via extracellular signal regulated kinases ½. Oncotarget. 9, 6814-6829 (2018). 29467931 |
| Protein Range | 3rd Intracellular Domain |
| Gene Symbol | ADORA2A |
|---|---|
| Alias Symbols | ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8, AA2AR, A2AR |
| NCBI Gene Id | 135 |
| Uniprot ID | P29274 |
| Protein Accession # | NP_000666.2 |
| Nucleotide Accession # | NM_000675 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ADORA2A Antibody (OALA02443)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Rabbit, Mouse, Dog, Guinea pig, Rat, Human".
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How long will it take to receive "ADORA2A Antibody (OALA02443)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "ADORA2A Antibody (OALA02443)" provided in?
This item is provided in "Liquid0.01M Tris-Glycine (pH 7.4), 0.15M sodium chloride, 0.05% sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ADORA2A Antibody (OALA02443)"?
This target may also be called "ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8, AA2AR, A2AR" in publications.
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What is the shipping cost for "ADORA2A Antibody (OALA02443)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ADORA2A Antibody (OALA02443)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ADORA2A Antibody (OALA02443)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ADORA2A Antibody (OALA02443)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ADORA2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ADORA2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ADORA2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ADORA2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ADORA2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ADORA2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
