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Catalog No: OKEH07827
Size:96T
Price: $800.00
SKU
OKEH07827
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ADORA1 ELISA Kit (Bovine) (OKEH07827)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology ADORA1 ELISA Kit (Bovine) (OKEH07827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADORA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADORA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADORA1 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.8% (n = 20)
Mean Inter-assay CV%: <=11.9% (n = 20)
ELISA Kit Component
ComponentAmount
ADORA1 Microplate96 Wells (12 x 8 Well strips)
ADORA1 Lyophilized Standard2
100X Biotinylated ADORA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
::Subcellular Location: Cell membrane Multi-pass membrane protein
::Unigene: Bt.76442
SMR: P28190
String: 9913.ENSBTAP00000015230
::KEGG: bta:282133
Pfam: PF00001
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine Adenosine receptor A1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADORA1
Gene Full Nameadenosine A1 receptor
Alias Symbolsadenosine receptor A1.
NCBI Gene Id282133
Protein NameP28190
Description of TargetAdenosine receptor A1
Uniprot IDhttps://www.uniprot.org/uniprot/P28190
Protein Accession #NP_776922.2
Nucleotide Accession #NM_174497.3
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